Antiangiogenic Activity of Mangostins Isolated from Thailand Stingless Bee Propolis

Background/Objective Propolis, a resinous substance collected by honeybees from origin plants to protect their beehive, has been reported have various biological activities and gained popularity as an alternative medicine in recent years. We previously the plant origin, chemical composition some of native Thailand stingless bee propolis. In this study, we further elucidated its active components mode action. Methods/Results Among nine prenylated xanthones isolated propolis, α-mangostin γ-mangostin were shown strongly inhibit tube formation human umbilical vein endothelial cells (HUVECs), indication antiangiogenic activity vitro. Further investigation effects at molecular level revealed that both mangostins inactivated extracellular signal-regulated kinase 1/2 (ERK1/2) activated p38 tube-forming HUVECs concentration-dependent manner. Taken together, two angiogenesis vitro inactivating angiogenic signal ERK1/2 activating stress p38. Detailed comparison seemed slightly stronger effect than these experiments. Combined with fact being most abundant component among tested, might be responsible for Conclusion These results indicate propolis may good candidate future applications prevention angiogenesis-related diseases.